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Detection of HLA Alleles Associated with Diseases

The Department of HLA of the Institute of Hematology and Blood Transfusion (IHBT) organizes quality control “Detection of HLA alleles associated with the diseases” since 2010.

This quality control is included among the international proficiency testing programs of the European Federation for Immunogenetics.

We offer the following options:

  1. B*27 (association with Morbus Bechterev and other rheumatoid autoimmune diseases)
  2. Alleles of DQ loci associated with coeliac disease (DQA1*02, *03, *051, DQB1*02, *03:02)
  3. DQB1*06:02 (association with narcolepsy)

Results obtained from laboratories are compared with the complete genotyping done by the organizer using standard operation procedures accredited by the Czech Accreditation Institute and the European Federation for Immunogenetics. For successful attendance the participants have to be consistent with the organizer in ≥ 90% in genotyping HLA alleles and ≥ 90% in interpreting results in association to the disease. All results and their evaluation are summarized in a final report. Each laboratory receives a certificate of participation.

Guarantor: Ing. Milena Vraná

(NRL for DNA diagnostics ÚHKT, U Nemocnice 1, 128 20 Praha 2, milena.vrana@uhkt.cz)

Organization:

Anonymous DNA samples required for MPZ variants in sealed test tubes with the identification of DNA are sent to all participants who filled in the application form. Each tube contains 50 μl of DNA of given concentration. At the same time, an invoice for payment and an electronic form for recording the results are sent to the participants.

 

Recommendations for the interpretation of HLA tests for the diagnosis of coeliac disease you can find in paper:

Harmonisation of the HLA tests for the diagnosis of coeliac disease: experiences from the Czech external proficiency testing program.

Front. Genet. 15:1441769.

doi: 10.3389/fgene.2024.1441769

Link to the article

 

Information on the currently ongoing EPT:

Dear colleagues,

as in previous years, in 2025 we will again organize external proficiency testing for “HLA association with diseases”. Information on the organization will be published here during January/February 2025.

Please note that the procedure for evaluating the results has changed and is described in the Recommendations for the diagnosis of HLA predisposition to celiac disease, which can be found below.