Department of Proteomics

The Department of Proteomics focuses on properties and functions of cellular proteins in relation to the origin, development and therapy of hematological diseases.

We aim to understand specific molecular mechanisms involved in regulation of normal and pathological hematopoiesis as well as in the effects of established or experimental therapeutical procedures. Basic techniques of cell and molecular biology (flow cytometry, western blot, immunofluorescence microscopy, molecular cloning) are used in combination with other methods and approaches to get a comprehensive view of the protein behavior.

Besides performing projects of basic and applied research, we also assist in introduction of novel diagnostic methods for hematology.

• We participated in the Independent Validation Initiative at antibodies-online and validated the Nucleophosmin antibody. See the results.

Main current topics

• Mechanisms of chemotherapy-induced changes in apoptosis-related proteins
• Consequences of C-terminal nucleophosmin mutations in acute myeloid leukemia
• Mechanisms of immune escape in acute myeloid leukemia (AML)
• Exosomes, proteomics, and cell surface markers in CML
• Screening of therapeutic targets in chronic myeloid leukemia cells resistant to general treatment
• Interaction of hematopoietic cells with the extracellular matrix
• Regulation of cell adhesion signaling in B-ALL immunotherapy