Laboratory of Flow Cytometry

The laboratory is equipped with three flow cytometers (BD FACSCalibur, BD FACSCanto I, BD FACSCanto II) and one cell sorter (BD FACSAria III). All methods (SOPs) are established in agreement with validated guidelines of the diagnostic platform of the European LeukemiaNet (ELN), International Clinical Cytometry Society (ICCS), and European Society of Clinical Cell Analysis (ESCCA) and accredited according to ČSN EN ISO 15189.

Hemato-oncological investigation includes the diagnosis and follow up of acute myeloid (AML), acute lymphoblastic (ALL) leukemias, myelodysplastic syndrome (MDS), lymphoproliferative disorders as well as minimal residual disease (MRD) detection in AML, ALL, B-CLL, MM and B- LPD.

Investigation of non-malignant hematological disorders includes the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH), inherited and acquired platelet disorders, differential diagnosis of heparin induced thrombocytopenia (HIT) as well as atypical hemolytic uremic syndrome (aHUS). For the needs of transplantation medicine, we perform quantitative and cell function analysis of CMV specific memory T cells.

The laboratory participates in a quality proficiency testing scheme (UK NEQAS) for the following programs: paroxysmal nocturnal hemoglobinuria, PNH high resolution, leukemia immunophenotyping, diagnostic interpretation and minimal residual disease.