Úsek pro vědu a výzkum

Ostatní diagnostické metody

Pro HPV diagnostiku metodami PCR používáme tyto sety primerů:


1 HPV typy spojené s epidermodysplasia verruciformis 

Literatura:

A) Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinski SM. Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cell/ Molecular diagnostics of human cancer 1989; 7: 209-14.

I) Hildesheim A, Schiffman MH, Gravitt P, Glass AG, Greer CE, Zhang T, Scott B, Rush BB, Lawler P, Sherman ME, Kurman RJ, manos MM. Persistence of type-specific human papillomavirus infection among cytologically normal women. J Inf Dis 1994; 169: 235-240.

B) de Roda Husman AM, Walboolmers JM, van den Brule AJ, Meijer CJ, Snijders PJ. The use of general primers GP5 and GP6 elongated at their 3' ends with adjacent highly conserved sequences improves human papillomavirus detection by PCR. J Gen Virol 1995; 76:1057-62. 
PMID: 9049358

C) van den Brule AJ, Pol R, Fransen-Daalmeijer N, Schouls LM, Meijer CJ, Snijders PJ. GP5+/6+ PCR followed by reverse line blot analysis enables rapid and high-throughput identification of human papillomavirus genotypes. J Clin Microbiol. 2002; 3:779-87. 
PMID: 11880393

E) Forslund O, Antonsson A, Nordin P, Stenquist B, Hansson BG. A broad range of human papillomavirus types detected with a general PCR method suitable for analysis of cutaneous tumours and normal skin. J Gen Virol 1999; 80: 2437-43. 
PMID: 10501499

F) Berkhout RJ, Tieben LM, Smits HL, Bavinck JN, Vermeer BJ, ter Schegget J. Nested PCR approach for detection and typing of epidermodysplasia verruciformis-associated human papillomavirus types in cutaneous cancers from renal transplant recipients. J Clin Microbiol 1995; 33(3): 690-5. 
PMID: 7751378

H) Gravitt PE, Peyton CL, Alessi TQ, Wheeler CM, Coutlee F, Hildesheim A, Schiffman MH, Scott DR, Apple RJ. Improved amplification of genital human papillomaviruses. J Clin Microbiol 2000; 38(1): 357-61. 
PMID: 10618116

D) Kleter B, van Doorn L-J, ter Schegget J, et al. Novel short-fragment PCR assay for highly sensitive broad-spectrum detection of anogenital human papillomaviruses. Am J Pathol 1998; 153: 1731-9.
PMID: 9846964