National Study of Mutations, Clonality and DNA Methylation in Patients With Acute Myeloid Leukemia – Collaborative Project with Faculty Hospital Brno

The main goal of the project is a mutational analysis of the wide spectrum of genes in a large cohort of the AML patients and determination of the detected mutations’ frequency, type, mutual coexistence, clonality and stability in the course of the disease. Based on the comparison with clinical data, we will be able to design a broadened classification for the prognostic/risk groups as well as to determine new markers for minimal residual disease monitoring.

Apart from this, we aim to reveal novel genes, which are consistently deregulated by aberrant DNA methylation in AML with defined mutational background and determine the affected pathways. DNA methylation is a reversible process, therefore the knowledge of the location and extent of aberrant DNA methylation represents a great potential for therapy. The link between gene expression and DNA methylation data is also needed to find pathologically relevant DNA methylation changes.